Built-In Self-Test Quality Assessment Using Hardware Fault Emulation in FPGAs

This paper addresses the problem of test quality assessment, namely of BIST solutions, implemented in FPGA and/or in ASIC, through Hardware Fault Emulation (HFE). A novel HFE methodology and tool is proposed, that, using partial reconfiguration, efficiently measures the quality of the BIST solution. The proposed HFE methodology uses Look-Up Tables (LUTs) fault models and is performed using local partial reconfiguration for fault injection on Xilinx(TM) Virtex and/or Spartan FPGA components, with small binary files. For ASIC cores, HFE is used to validate test vector selection to achieve high fault coverage on the physical structure. The methodology is fully automated. Results on ISCAS benchmarks and on an ARM core show that HFE can be orders of magnitude faster than software fault simulation or fully reconfigurable hardware fault emulation

Transformação de algodão (Gossypium Hirsutum L.) através do uso de policátion.

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Aulas de Ciências pautadas nas Unidades de Ensino Potencialmente Significativas sobre o tema Água

Apresentamos uma utilização das Unidades de Ensino Potencialmente Significativa (UEPS) como metodologia para aulas de ciências. São abordadas questões inseridas no tema água, em ambientes não formais de ensino, visando à promoção de aulas mais contextualizadas. Dessa forma pretende-se diminuir a distância entre o cotidiano dos alunos e os conteúdos de Ciências, ao promover a alfabetização científica e a inserção da educação ambiental. Foi elaborada uma sequência de ensino baseada nos 8 passos da UEPS e aplicada a uma turma do 9º ano do Ensino Fundamental de uma escola estadual. Para avaliar a viabilidade desta proposta foram coletados dados através de questionários antes e após as aulas e observação. Estes dados foram analisados através da análise textual discursiva. Constatou-se que as aulas tiveram uma excelente aceitação por parte dos alunos, pois, relataram que a aprendizagem foi mais efetiva quando comparada às aulas tradicionais

Decreasing Mortality in Severe Sepsis and Septic Shock Patients by Implementing a Sepsis Bundle in a Hospital Setting

Background The Surviving Sepsis Campaign (SSC) guidelines for the management of severe sepsis (SS) and septic shock (SSh) have been recommended to reduce morbidity and mortality. Materials and Methods A quasi-experimental study was conducted in a medical-surgical ICU. Multiple interventions to optimize SS and SSh shock patients\u27 clinical outcomes were performed by applying sepsis bundles (6- and 24-hour) in May 2006. We compared bundle compliance and patient outcomes before (July 2005-April 2006) and after (May 2006-December 2009) implementation of the interventions. Results A total of 564 SS and SSh patients were identified. Prior to the intervention, compliance with the 6 hour-sepsis resuscitation bundle was only 6%. After the intervention, compliance was as follows: 8.2% from May to December 2006, 9.3% in 2007, 21.1% in 2008 and 13.7% in 2009. For the 24 hour-management bundle, baseline compliance was 15.0%. After the intervention, compliance was 15.1% from May to December 2006, 21.4% in 2007, 27.8% in 2008 and 44.4% in 2009. The in-hospital mortality was 54.0% from July 2005 to April 2006, 41.1% from May to December 2006, 39.3% in 2007, 41.4% in 2008 and 16.2% in 2009. Conclusion These results suggest reducing SS and SSh patient mortality is a complex process that involves multiple performance measures and interventions

Impact of Appropriate Antimicrobial Therapy for Patients with Severe Sepsis and Septic Shock – A Quality Improvement Study

Background There is ample literature available on the association between both time to antibiotics and appropriateness of antibiotics and clinical outcomes from sepsis. In fact, the current state of debate surrounds the balance to be struck between prompt empirical therapy and care in the choice of appropriate antibiotics (both in terms of the susceptibility of infecting organism and minimizing resistance arising from use of broad-spectrum agents). The objective of this study is to determine sepsis bundle compliance and the appropriateness of antimicrobial therapy in patients with severe sepsis and septic shock and its impact on outcomes. Material This study was conducted in the ICU of a tertiary care, private hospital in São Paulo, Brazil. A retrospective cohort study was conducted from July 2005 to December 2012 in patients with severe sepsis and septic shock. Results A total of 1,279 patients were identified with severe sepsis and septic shock, of which 358 (32.1%) had bloodstream infection (BSI). The inpatient mortality rate was 29%. In evaluation of the sepsis bundle, over time there was a progressive increase in serum arterial lactate collection, obtaining blood cultures prior to antibiotic administration, administration of broad-spectrum antibiotics within 1 hour, and administration of appropriate antimicrobials, with statistically significant differences in the later years of the study. We also observed a significant decrease in mortality. In patients with bloodstream infection, after adjustment for other covariates the administration of appropriate antimicrobial therapy was associated with a decrease in mortality in patients with severe sepsis and septic shock (p = 0.023). Conclusions The administration of appropriate antimicrobial therapy was independently associated with a decline in mortality in patients with severe sepsis and septic shock due to bloodstream infection. As protocol adherence increased over time, the crude mortality rate decreased, which reinforces the need to implement institutional guidelines and monitor appropriate antimicrobial therapy compliance

A program for sustained improvement in preventing ventilator associated pneumonia in an intensive care setting

Background Ventilator-associated pneumonia (VAP) is a common infection in the intensive care unit (ICU) and associated with a high mortality. Methods A quasi-experimental study was conducted in a medical-surgical ICU. Multiple interventions to optimize VAP prevention were performed from October 2008 to December 2010. All of these processes, including the Institute for Healthcare Improvement’s (IHI) ventilator bundle plus oral decontamination with chlorhexidine and continuous aspiration of subglottic secretions (CASS), were adopted for patients undergoing mechanical ventilation. Results We evaluated a total of 21,984 patient-days, and a total of 6,052 ventilator-days (ventilator utilization rate of 0.27). We found VAP rates of 1.3 and 2.0 per 1,000 ventilator days respectively in 2009 and 2010, achieving zero incidence of VAP several times during 12 months, whenever VAP bundle compliance was over 90%. Conclusion These results suggest that it is possible to reduce VAP rates to near zero and sustain these rates, but it requires a complex process involving multiple performance measures and interventions that must be permanently monitored

Assessment of Cytotoxic Activity of Rosemary ( Rosmarinus officinalis

The objective of this study was to evaluate the cytotoxic activity of rosemary (REO, Rosmarinus officinalis L.), turmeric (CEO, Curcuma longa L.), and ginger (GEO, Zingiber officinale R.) essential oils in HeLa cells. Cytotoxicity tests were performed in vitro, using tetrazolium (MTT) and neutral red assays for evaluation of antiproliferative activity by different mechanisms, trypan blue assay to assess cell viability and evaluation of cell morphology for Giemsa to observe the cell damage, and Annexin V to evaluate cell death by apoptosis. CEO and GEO exhibited potent cytotoxic activity against HeLa cells. IC50 obtained was 36.6 μg/mL for CEO and 129.9 μg/mL for GEO. The morphology of HeLa cells showed condensation of chromatin, loss of cell membrane integrity with protrusions (blebs), and cell content leakage for cells treated with CEO and GEO, from the lowest concentrations studied, 32.81 μg/mL of CEO and 32.12 μg/mL of GEO. The Annexin V assay revealed a profile of cell death by apoptosis for both CEO and GEO. The results indicate cytotoxic activity in vitro for CEO and GEO, suggesting potential use as anticancer agents for cervical cancer cells

Evaluation of genetic variation among Brazilian soybean cultivars through genome resequencing.

Soybean [Glycine max (L.) Merrill] is one of the most important legumes cultivated worldwide, and Brazil is one of the main producers of this crop. Since the sequencing of its reference genome, interest in structural and allelic variations of cultivated and wild soybean germplasm has grown. To investigate the genetics of the Brazilian soybean germplasm, we selected soybean cultivars based on the year of commercialization, geographical region and maturity group and resequenced their genomes. We resequenced the genomes of 28 Brazilian soybean cultivars with an average genome coverage of 14.8X. A total of 5,835,185 single nucleotide polymorphisms (SNPs) and 1,329,844 InDels were identified across the 20 soybean chromosomes, with 541,762 SNPs, 98,922 InDels and 1,093 CNVs that were exclusive to the 28 Brazilian cultivars. In addition, 668 allelic variations of 327 genes were shared among all of the Brazilian cultivars, including genes related to DNA-dependent transcription-elongation, photosynthesis, ATP synthesis-coupled electron transport, cellular respiration, and precursors of metabolite generation and energy. A very homogeneous structure was also observed for the Brazilian soybean germplasm, and we observed 41 regions putatively influenced by positive selection. Finally, we detected 3,880 regions with copy-number variations (CNVs) that could help to explain the divergence among the accessions evaluated. The large number of allelic and structural variations identified in this study can be used in marker-assisted selection programs to detect unique SNPs for cultivar fingerprinting. The results presented here suggest that despite the diversification of modern Brazilian cultivars, the soybean germplasm remains very narrow because of the large number of genome regions that exhibit low diversity. These results emphasize the need to introduce new alleles to increase the genetic diversity of the Brazilian germplasm

Plantas medicinais amazônicas Leishmanicidas (parte I).

Leishmaniose é uma doença altamente difundida pelo mundo, ocorrendo da Ásia até as Américas. Em razão de grandes alterações ambientais causadas pelo homem, cada vez mais o número de registros desta doença cresce em países tropicais. A leishmaniose está na lista de doenças negligenciadas devido à baixos investimentos em pesquisas laboratoriais e tratamento para a população menos favorecida. Assim, uma das alternativas utilizadas pelas populações é o uso de plantas medicinais no tratamento contra esta doença. Neste sentido, este trabalho teve como objetivo realizar o levantamento de plantas medicinais amazônicas utilizadas no tratamento contra a leishmaniose. Para o início de pesquisa, foi realizado um levantamento bibliográfico nos bancos de dados disponíveis, onde foram procuradas espécies, nome vernacular e modo de uso e para complementar foi acrescentado a parte utilizada do vegetal. Após o levantamento, foram encontradas 13 plantas medicinais que ocorrem na Amazônia com relato para o efeito leishmanicida. Com isso, a pesquisa permitiu analisar quais as espécies são utilizadas no tratamento de leishmaniose auxiliando estudos fitoquímicos, farmacológicos e de validação toxicológica. Portanto, este estudo deve ter continuidade afim de se obter outras plantas que apresente característica leishmanicida.Publicado também como resumo